The Medimachine II is designed to provide both gentle tissue dissociation and fine homogenization using appropriate disposable devices with different technical features according to the required procedure.
The instrument has an interactive touch-screen for selection of operation mode and for consulting the on board manual. This specific screen has been selected for use wearing lab gloves. The software offers multi-lingual settings and two modes: manual and automatic.
The Medimachine II Dissociator is a benchtop instrument for the automated dissociation of tissues to obtain single-cell suspensions. Single-cell suspensions are a crucial prerequisite to achieve reliable results in cell separation, cell analysis, and cell culture. Moreover, the Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications.
Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias, etc.
Four types of disposables have been specially designed:
A number of Medimachine II programs have been specifically developed to offer standardized procedures for the gentle dissociation or fine homogenization of various samples. Operator could add and remove new customized programs.
|CE Certified||Conform to UL / CSA|
|Size||211 mm x 164 mm x 257 mm|
Medimachine II System
Medimachine II System
The Medimachine system is a sample preparation system for the automated, mechanical disaggregation of solid animal or plant tissues for flow cytometric analysis, cell culture, or DNA amplification.
The Medimachine system consists of three components: the Medimachine, Medicons, and Filcons. Various pore sizes of disposable, sterile, and non-sterile Medicons and Filcons allow the operator to optimize experimental conditions to suit the tissue under study, ensuring clean, viable preparations of single cells, cell clusters, or cell nuclei. The procedure is fast and simple.
An increasing number of laboratories are performing flow cytometric analyses of solid tissues and tumours for antigen expression, ploidy, and proliferative index. The quality of the final result in the flow cytometric analysis of any tissue depends upon the quality of the prepared suspension. Various manual methods for dissociating tissue have been used alone or in combination and include:
These methods can be time consuming, and all have limitations with regard to operator safety and the quality of the final cell suspension. Tissue composition must be considered, since some tissues disaggregate more readily than others, and abnormal tissue might contain very delicate cells. Thus, differences in technicians’ skills and technique can result in low cell viability and yield, as well as poor reproducibility.
|Supply:||82/250 Vac 60/50 Hz. (switching)|
|Dimensions:||cm. 20 x 15 x 15|
The following is an overview of the steps for using the Medimachine system for typical tissue disaggregation. The procedure should be adapted for your laboratory and each tissue type you receive. A small piece of tissue (up to 10 mm3), free of fat and necrosis, is inserted into the Medicons together with ~1.0 mL of the desired suspension buffer (for example, PBS).
The Medicons is then inserted into the Medimachine and the machine is started. The machine can be run from 10 seconds to 4 minutes depending upon the tissue type and the cell suspension you want.
As the tissue is disaggregated, the cells pass through the screen into the suspension liquid in the bottom of the Medicon (Figures 1 and 2).
Once the tissue is disaggregated, the Medicons is removed from the Medimachine. A needless syringe is then inserted in the syringe port to recover the cell suspension. The Medicons may be rinsed with suspension buffer to ensure maximum cell recovery (Figure 3). Select the appropriate Filcons pore size for isolation of the desired cells (Figure 4).
The Medimax system is a sample preparation system for the automated, mechanical disaggregation of solid animal or plant tissues for flow cytometric analysis, cell culture, or DNA amplification techniques.
The Medimax system makes tissue preparation safe and independent of operator technique, and facilitates standardizing the preparation of all tissue types. Times and protocols can even be standardized across labs.
The Medimax system consists of three components:
The compact Medimax unit is the heart of the system. It works with all types of Medicons and operates at a constant speed of approximately 80 rpm.
|Supply:||82/250 Vac 60/50 Hz. (switching)|
|Dimensions:||cm. 22 x 25 x 20|
A small piece of tissue (up to 10 mm3), free of fat and necrosis, is inserted into the Medicons together with the correct amount of liquid (~1.0 mL for codes 79300 and 79400; ~16.0 mL for code 79500) of the desired suspension buffer (for example, PBS)
The Medicons is then inserted into the Medimax and the machine is started. The machine can be run from 10 seconds to 4 minutes depending upon the tissue type and the cell suspension you want. As the tissue is disaggregated, the cells pass through the screen into the suspension liquid in the bottom of the Medicons. Once the tissue is dissociated, the Medicons is removed from the Medimax. A needleless syringe is then inserted in the syringe port to recover the cell suspension. The Medicons may be rinsed with suspension buffer to ensure maximum cell recovery. Select the appropriate Filcons (or Filcons-Max if a 50 mL tube is used) pore size for isolation of the desired cells.
Medicons are disposable polyethylene chambers containing an immobile stainless steel screen with approximately 100 hexagonal holes. Around each hole are six microblades designed for efficient cutting of hard and soft tissues
A stainless steel element rotates on the screen, bringing the tissue to the blades and letting the gently dissociated parts pass through the bore-holes. A micropump positioned under the screen makes sure that the liquid passes through the bore-holes, maintaining them constantly clean. Disaggregation of the sample takes from 30 seconds up to 4 minutes. Small quantities of tissue are sufficient because the recovery rate is very high.
|Medicons Code:||79300 SN / S - 79400 SN / S|
|Medicons Max Code:||79500 SN - 79500 S|
|A35 μm||Recovery of cell sizes up to 160 μm|
|50 μm||Recovery of cell sizes up to 230 μm|
|Never place the tissue into a dry Medicons|
|Do not overfill the Medicons with suspension buffer. This will cause the tissue to float and can result in inadequate disaggregation: dilution must be between 1 and 1,2 mL for standard Medicons (codes 79300 and 79400) and between 15 and 16 mL for Large Medicons (code 79500).|
|If the amount of tissue is very small, the suspension buffer can be replaced with staining solution for maximum cell recovery.|
|As the rotating element passes over the microblades, it tends to blunt the edges, reducing the cutting efficiency.|
|Do not reuse the Medicons for successive samples.|
|Large Medicons (big white) is available in 50 μm mesh size, while Medicons standard (green and orange) are available (sterile or not sterile) in two mesh sizes.|
|Before use, wet the Filcons filter membrane with cell suspension buffer to reduce the surface tension and facilitate the filtration process.|
|Isolation of nuclei, cells, and cell clusters can be achieved by varying the Filcons pore size.|